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【转载】PH4CV专利描述原文节选  

2014-05-16 19:15:59|  分类: 默认分类 |  标签: |举报 |字号 订阅

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本文转载自时寒心《PH4CV专利描述原文节选》
United States Patent 6,897,363
Barker May 24, 2005

Inbred maize line PH4CV

Abstract

An inbred maize line, designated PH4CV, the plants and seeds of inbred maize line PH4CV, methods for producing a maize plant, either inbred or hybrid, produced by crossing the inbred maize line PH4CV with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH4CV with another maize line or plant and to methods for producing a maize plant containing in its genetic material one or more transgenes and to the transgenic maize plants produced by that method. This invention also relates to inbred maize lines derived from inbred maize line PH4CV, to methods for producing other inbred maize lines derived from inbred maize line PH4CV and to the inbred maize lines derived by the use of those methods.


Inventors: Barker; Thomas Charles (York, NE)
Assignee: Pioneer Hi-Bred International, Inc. (Johnston, IA)
Appl. No.: 10/271,942
Filed: October 15, 2002

Current U.S. Class: 800/320.1 ; 435/412; 800/275; 800/278; 800/279; 800/281; 800/298; 800/300.1; 800/301; 800/302; 800/303
Current International Class: A01H 5/10 (20060101); A01H 001/00 (); A01H 004/00 (); A01H 005/00 (); A01H 005/10 (); C12N 015/82 ()
Field of Search: 800/320.1,266,275,278,279,284,298,300.1,301,302,303 435/412


References Cited [Referenced By]

U.S. Patent Documents
5436390 July 1995 Oestreich
5563325 October 1996 Morrow
5792911 August 1998 Anderson
5850010 December 1998 Anderson
6333453 December 2001 Henke

Other References

Plant Variety Protection Certificate No. 9500200 for Corn PHBE2, issued Aug. 13, 1996. .
Plant Variety Protection Certificate No. 9100097 for Corn PHRO3, issued Apr. 30, 1992. .
Plant Variety Protection Certificate No. 200000242 for Corn, Field PH1BC, issued Jan. 13, 2002. .
Plant Variety Protection Certificate No. 200000243 for Corn, Field PH2EJ, issued Feb., 5, 2002. .
Plant Variety Protection Certificate No. 9600200 for Corn PH56C, issued Jul. 30, 1999. .
Plant Variety Protection Certificate No. 9700223 for Corn, Field, PH24M, issued Apr., 24, 2001..

Primary Examiner: Kruse; David H
Attorney, Agent or Firm: McKee, Voorhees & Sease, P.L.C.

Parent Case Text



CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims benefit of the priority date of U.S. Patent Application Ser. No. 60/352,317 filed Jan. 28, 2002, which is hereby incorporated herein by reference.
Claims



What is claimed is:

1. A seed of maize inbred line designated PH4CV, representative seed of said line having been deposited under ATCC Accession No. PTA-4673.

2. A maize plant, or a part thereof, produced by growing the seed of claim 1.

3. The maize plant of claim 2, wherein said plant has been detasseled.

4. A tissue culture of regenerable cells produced from the plant of claim 2.

5. A protoplast produced from the tissue culture of claim 4.

6. The tissue culture of claim 4, wherein cells of the tissue culture are produced from a tissue selected from the group consisting of leaf, pollen, embryo, root, root tip, anther, silk, flower, kernel, ear, cob, husk and stalk.

7. A maize plant regenerated from the tissue culture of claim 4, said plant having all the morphological and physiological characteristics of inbred line PH4CV, representative seed of said inbred line having been deposited under ATCC Accession No. PTA-4673.

8. A method for producing an F1 hybrid maize seed, comprising crossing the plant of claim 2 with a different maize plant and harvesting the resultant F1 hybrid maze seed.

9. A method of producing a male sterile maize plant comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers male sterility.

10. A male sterile maize plant produced by the method of claim 9.

11. A method of producing an herbicide resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers herbicide resistance.

12. An herbicide resistant maize plant produced by the method of claim 11.

13. The maize plant of claim 12, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

14. A method of producing an insect resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers insect resistance.

15. An insect resistant maize plant produced by the method of claim 14.

16. The maize plant of claim 15, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

17. A method of producing a disease resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers disease resistance.

18. A disease resistant maize plant produced by the method of claim 17.

19. A method of producing a maize plant with decreased phytate content comprising transforming the maize plant of claim 2 with a transgene encoding phytase.

20. A maize plant with decreased phytate content produced by the method of claim 19.

21. A method of producing a maize plant with modified fatty acid metabolism or modified carbohydrate metabolism comprising transforming the maize plant of claim 2 with a transgene encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase.

22. A maize plant, with modified fatty acid metabolism or modified carbohydrate metabolism, produced by the method of claim 21.

23. The maize plant of 22 wherein the transgene confers a trait selected from the group consisting of waxy starch and increased amylose starch.

24. A maize plant, or part thereof, having all the physiological and morphological characteristics of the inbred line PH4CV, representative seed of said inbred line having been deposited under ATCC Accession No. PTA-4673.

25. A method of introducing a desired trait into maize inbred line PH4CV comprising: (a) crossing PH4CV plants grown from PH4CV seed, representative seed of which has been deposited under ATCC Accession No. PTA-4673, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, disease resistance and waxy starch; (b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants; (c) crossing the selected progeny plants with the PH4CV plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

26. A plant produced by the method of claim 25, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

27. The plant of claim 26 wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

28. The plant of claim 26 wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.

29. The plant of claim 26 wherein the desired trait is male sterility and the trait is conferred by a cytoplasmic nucleic acid molecule that confers male sterility.


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